ahrC [2017-08-25 17:06:50]

transcriptional regulator of the ArgR family, AhrC represses the genes for arginine biosynthesis and activates the genes for arginine catabolism.

Locus: BSU24250

Isoelectric point: 5.52

Molecular weight: 16.69 kDa

Protein length: 149 aa

Gene length: 447 bp

Function: transcriptional regulator of arginine metabolic genes

Product: transcriptional regulator (ArgR family)

Essential: no

Synonyms: argR

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of arginine
• Metabolism → Amino acid/ nitrogen metabolism → Utilization of amino acids → Utilization of arginine/ ornithine
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

Gene

Coordinates: 2,522,324 → 2,522,773

Phenotypes of a mutant

• increased intracellular concentration of ornithine, citrulline, and arginine PubMed
• ahrC inactivation allows growth of a ktrA ktrB kimA mutant at low potassium concentration PubMed

The protein

Catalyzed reaction/ biological activity

• transcriptional activator/ repressor of genes involved in arginine metabolism

Protein family

• ArgR family

Cofactors

• L-arginine is the co-factor required for transcription repression/ activation

Structure

• 2P5M (C-Terminus), 2P5K (complex with an 18bp DNA operator), 2P5L (N-terminus), 1F9N, NCBI PubMed,N-Terminus NCBI, C-Terminus NCBI, complex with an 18bp DNA operator NCBI

Localization

• Cytoplasm

Expression and Regulation

Operons

• Genes: ahrC-recN
  Description: PubMed

  Regulation

  • by sRNA sr1

  Additional information

  • expression is fourfold increased upon depletion of nusA Reference

• Genes: dxs-yqxC-ahrC-recN
  Description: PubMed

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

Other regulations

• Sr1: translation inhibition,

Biological materials

Mutant

• GP729 (ahrC::aphA3), available in Jörg Stülke's lab
• GP2185 (ΔahrC::ermC), available in Jörg Stülke's lab PubMed

Labs working on this gene/protein

• Simon Phillips, Leeds University, UK Homepage
• Michel Debarbouille, Pasteur Institute, Paris, France Homepage

References

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